NEET Biology — Fundamentals
Cell Biology (High Priority)
Cell Structure:
Prokaryotic: No membrane-bound nucleus, no membrane organelles
Examples: bacteria, blue-green algae (cyanobacteria)
70S ribosomes, circular DNA (no histones), cell wall of peptidoglycan
Eukaryotic: Membrane-bound nucleus, membrane organelles
Examples: plant cells, animal cells, fungi, protists
80S ribosomes (except mitochondria/chloroplast — 70S)
Linear DNA with histones
Key organelles:
Mitochondria: "Powerhouse" — ATP synthesis (oxidative phosphorylation)
Double membrane, own DNA, 70S ribosomes
Number highest in cells with high energy needs (liver, muscle)
Chloroplast: Photosynthesis — light reactions in thylakoid, dark in stroma
Double membrane, own DNA, 70S ribosomes
Only in plant cells and algae
Ribosome: Protein synthesis (translation)
80S (cytoplasm) = 60S + 40S subunits
70S (mitochondria, chloroplast, prokaryotes) = 50S + 30S
Golgi complex: Post-translational modification, packaging, secretion
Cis face (receives from ER) → Trans face (ships out)
Makes cell wall material in plants
Lysosome: "Suicide bag" — digestive enzymes, intracellular digestion
Only in animal cells (NOT plant cells)
pH 5 (acidic) — optimal for hydrolytic enzymes
Nucleus: Contains DNA, site of transcription
Double membrane with nuclear pores
Nucleolus: rRNA synthesis
Photosynthesis
Overall equation: 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂ (light energy)
Light Reactions (Thylakoid membrane):
PS II (P680) → absorbs light, water splits (O₂ released), electrons flow
Electron transport chain → creates proton gradient
PS I (P700) → excited again, NADP⁺ reduced to NADPH
ATP synthase → ADP + Pi → ATP (photophosphorylation)
Output: ATP + NADPH + O₂
Calvin Cycle / Dark Reactions (Stroma):
CO₂ fixation: CO₂ + RuBP → 2 × 3-PGA (enzyme: RuBisCO)
Reduction: 3-PGA → G3P (uses ATP + NADPH)
Regeneration: G3P → RuBP (uses ATP)
Net: 3CO₂ → 1 molecule G3P (need 6 turns for 1 glucose)
C₃ plants: 3-PGA (3-carbon compound) first stable product
Examples: wheat, rice, potato, sunflower
More common, less efficient in hot/dry conditions
C₄ plants: OAA (4-carbon) first product, Kranz anatomy
Examples: maize, sugarcane, sorghum
Minimal photorespiration, efficient in hot/sunlight
CAM plants: Stomata open at night, CO₂ stored as malate
Examples: cacti, pineapple, agave
Extreme drought adaptation
Human Physiology — Digestion
Mouth: Salivary amylase (ptyalin) → starch to maltose (pH 6.8)
Mucus lubricates food; chewing breaks down mechanically
Stomach: HCl (pH 1-2) activates pepsinogen → pepsin
Pepsin: proteins → peptides
Gastric lipase: fats (minor digestion)
Chyme = acidic food mass leaving stomach
Small Intestine (main absorption site):
Duodenum: bile (liver, stored in gall bladder) emulsifies fats
pancreatic juice: amylase, lipase, trypsin, chymotrypsin
Jejunum + Ileum: villi and microvilli (brush border) → maximum absorption
Final enzymes: maltase, lactase, sucrase (disaccharidases)
Peptidases → amino acids
Amino acids + glucose + water → blood (portal vein)
Fatty acids + glycerol → lacteals (lymph)
Large Intestine:
Absorption of water, minerals
Formation of feces
Bacteria produce Vitamin K, Vitamin B₁₂